Mario and Luigi find Yoshi after the Princess was kidnapped by Bowser in Dinosaur land, the three go on a quest to rescue Yoshi's friends from the Koopalings, and the Princess from Bowser, after Mario defeats him, the four plus the eggs travel back to Yoshi's house.
- Big Boo
- Iggy Koopa
- Morton Koopa Jr.
- Lemmy Koopa
- Ludwig Von Koopa
- Roy Koopa
- Wendy O. Koopa
- Larry Koopa
- Yoshi's hands are orange in this game.
- The Koopalings are all different colors in this game.
- This is the first game where each individual level is specifically named.